Coding

Part:BBa_K831000:Design

Designed by: Silvia J Cañas   Group: iGEM12_Colombia   (2012-09-26)


HipA7 toxin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Due to its toxicity of HipA7, constructs for expression of this toxin in a E. coli chassis must be made using a inducible promoter with low leakage.


Source

This part was extracted of the genome of Escherichia coli K12 TH1269 using high fidelity PCR

References